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Fluorescence microscopy
7 cards·by Leos
Properties of fluorochomes
1. Brightness, 2. Spectrum, 3. Stability
answer
Confocal
Out of focus light is reflected because it does not pass the pinhole in front of the detector
Multifocal
Multiple lasers and detectors
Multi Photon Excitation
Excitation with 2x excitation wavelength -> deeper into sample because of higher
wavelength. You need a lot of light because of low energy
Total Internal Reflection Fluorescence
Light is totally reflected -> formation of evanescent wave directly under surface.
Elimination of background noise
Tagged protein imaging
Tracking fluorescence or measure fluorescence modulation (e.g. FRET)